hTERT activation of human bone marrow mesenchymal stem cells telomerase activity in.docVIP

 PAGE \* MERGEFORMAT 26 hTERT activation of human bone marrow mesenchymal stem cells telomerase activity in Abstract clearly exogenous hTERT (human telomerase reverse transcriptase, hTERT) could activate the human bone marrow-derived mesenchymal stem cells (human bone marrow mesenchyme stem cell, hBMSC) of telomerase activity. [Methods] cultured human bone marrow mesenchymal stem cells using lipofection method, the gene encoding hTERT eukaryotic expression plasmid pCIneo  hTERT into monoclonal cultured human bone marrow mesenchymal stem cells. The use of pressurized G418 selection, stable, with RT  PCR detection of hTERT mRNA expression, Western Blot protein expression was detected, special test kits, RT  PCR Detection of telomerase activity. [Results] The bone marrow-derived mesenchymal stem cells transfected with plasmid pCIneo  hTERT, the growth in good condition. Canadian G418 after 2 d, cells began to suspension, and gradually died. The first 10 d, non-transfected cells in the G418 group under the action of the death of all clones transfected group cells started proliferating. G418 concentrations down to 100 μg / ml to maintain selection, 20 d or so beginning to show significant anti-G418 cell clone. Transfected cells, the mRNA and protein expression of hTERT, telomerase activity was significantly increased at the same time. [Conclusion] The exogenous hTERT into human bone marrow mesenchymal stem cells can activate the cells, telomerase activity in immortalized cell lines for the establishment of the foundation. Keywords: human telomerase reverse transcriptase enzyme telomerase activity of human bone marrow mesenchymal stem cell gene Abstract: [Objective] To explicate whether the telomerase activity is regulated by human telomerase reverse transcriptase (hTERT) in human bone marrow mesenchyme stem cells (hBMSC). [Method] hBMSC were cultured and transfected with eukaryotic expressing plasmid pCIneo  hTERT encoding hTER