HPLC determination of anti-bone hyperplasia Icariin pills.docVIP

 PAGE \* MERGEFORMAT 7 HPLC determination of anti-bone hyperplasia Icariin pills [Abstract] To establish HPLC determination of anti-bone hyperplasia Icariin pills. Methods Diamonsil C18 (4.6mm * 200mm, 5μ m) column, mobile phase: acetonitrile - water (26:74), flow rate: 1.0ml/min, detection wavelength: 270nm. Results of icariin in 0.04052 ~ 0.5065μ g within the range of a good linear relationship between peak area, r = 0.99995, the average recovery was 99.4%, RSD of 0.6% (n = 6). Conclusion This measurement is simple, accurate, reproducible, and separation, and can be used for anti-bone hyperplasia quality control pills. [Keywords:] HPLC Epimedium Determination of anti-bone hyperplasia Pills [Abstract] Objective To establish a HPLC method for the determination of icariin in Kanggu zhizengsheng pills.Methods A DiamonsilTM C18 column (200mm * 4.6mm, 5μ m) was used with a mobile phase of acetonitrile-water (26:74). The flow rate was1.0ml * min-1 and the detection wavelength was 270nm. Results The linear range was at 0.04052? g ~ 0.5065? g (r = 0.99995) and the average recovery was 99.4% with RSD0.6% (n = 6) . Conclusion The determination method is simple and accurate and it can be used for the quality control of Kanggu zhizengsheng pills. [Keywords:] HPLC; icariin; Kanggu zhizengsheng pills; determination Anti-bone hyperplasia standard of Chinese medicine pill for the Ministry of Health set prescription drug formulations Volume containing relevant species [1], from Rehmannia glutinosa, Epimedium, Cistanche, etc. 9 Chinese herbal medicines, has a complement waist kidney, strong bones and muscles, promoting blood circulation, pain and other effects. For the treatment of hypertrophic spondylitis, cervical syndrome, bone spurs and other bone hyperplasia. None of the original standard assay methods, Fang Epimedium as the main Medicine Smell, its active ingredient is icariin. The method for determination of icariin, the main for the HPLC method [2,3]. In order t