HPLC Determination of Armosig Shu Lin Bartin axetil tablets in Human Plasma.docVIP

 PAGE \* MERGEFORMAT 4 HPLC Determination of Armosig Shu Lin Bartin axetil tablets in Human Plasma 【Abstract】 Objective To establish Armosig Shu Lin Bartin ester film amoxicillin and sulbactam plasma concentration determination. Methods The column was Spberiserb ODS (Φ3.9mm × 200mm, 5μm); mobile phase of acetonitrile-0.068mol / L KH2PO4 (4.5:95.5); flow rate of 0.8ml/min; detection limit of 225nm; column temperature was (25 ± 0.1) ℃ was determined in the human body plasma concentration of amoxicillin. In order to column was Hypersil C18 (Φ4.6mm × 200mm, 5μm); mobile phase of acetonitrile-0.068mol / L KH2PO4 (4.5:95.5); flow rate of 1.2ml/min; detection wavelength 210nm; column temperature ( 25 ± 0.1) ℃ sulbactam were measured in the human body plasma concentration. The results of amoxicillin and sulbactam in the selected chromatographic conditions, and the plasma impurity peaks separated well, separation is greater than 1.5. Amoxicillin plasma drug concentration in the range of 0.125 ~ 10μg/ml good linearity, r = 0.9998; sulbactam plasma drug concentration in the range of 0.10 ~ 10μg/ml good linearity, r = 0.9999. Of amoxicillin and sulbactam, the average recoveries were 96.25%, 102.3%. Two components of the day, day RSD lt;10%. Conclusion HPLC determination of amoxicillin and sulbactam Darfoner in vivo blood concentration method is accurate and a high degree of accuracy and sensitivity are consistent with measurement requirements of drug in plasma. Keywords: Armosig Shu Lin Bartin axetil tablets; high-performance liquid chromatography; blood drug concentration To detect human blood concentration of amoxicillin and sulbactam with HPLC 【Abstract】 Objective To set up the detection method of human blood concentration of amoxicillin and sulbactam. Methods The chromatographic column was Spberiserb ODS (φ3.9mm × 200mm.5μm), and the mobile phase was acetonitrile-0.068mol / L KH2PO4 (4.5 : 95.5), the flow rate was 0.8ml/min, the detect