HPLC determination of an only child rattan content in Tripterygium.docVIP

 PAGE \* MERGEFORMAT 10 HPLC determination of an only child rattan content in Tripterygium Of: Liu Huiqiong, Guocheng, Lu Qiwen, CONSERVATORY [Abstract] Objective To establish a high performance liquid chromatography son rattan content in Tripterygium for tripterine medicinal plants provide the basis for resource development. Methods Supelco Discovery C18 column (25 cm 4.6 mm, 5 m ), mobile phase was methanol -5% (by volume) H3PO4 solution (volume ratio 80:20), flow rate 1.0 mL min-1, the detection wavelength was 426 nm. Results tripterine in the 40 ~ 200 g mL -1 in the range of peak area was linear (r = 0.999 1). the only son in Tripterygium vines average of 5% mass fraction of 0.055, tripterine average recovery was 97.6%, RSD = 1.78 % (n = 6). Conclusion The method is simple, accurate, sensitive, reproducible, and can be used for an only child in Tripterygium vine Determination; only child in Tripterygium rattan higher levels can be used as triptolide red pigment to development and utilization of plant resources. [Keywords:] only child rattan; tripterine; high performance liquid chromatography; component analysis Abstract: ObjectiveTo determine the content of tripterine in Celastrus monospermus Roxb by HPLC for developing the medicinal resources of celastrol.Methods An external standard method by HPLC with Discovery C18 column (25 cm 4.6 mm, 5 m) was employed.The mobile phase was consisted of methanol-5% () phosphoric acid solution (80 ?U 20) () at the flow rate of 1.0 mL min-1.The detection wavelength was 426 nm.Results It showed good linearity in the range of 40 - 200 g mL-1 (r = 0.999 1). The content of tripterine in Celastrus monospermus Roxb was 0.055 5%, and the average recovery rate of celastrol was 97.6% (RSD = 1.78%, n = 6). Conclusion The method is simple and accurate, sensitive and reproducible and can be used in the quality control of Celastrus monospermus Roxb.The content of tripterine in Celastrus monospermus Roxb was high, whi