Human soluble vascular endothelial growth factor receptor 1 inhibited the proliferation of leukemic cells.docVIP

 PAGE \* MERGEFORMAT 13 Human soluble vascular endothelial growth factor receptor 1 inhibited the proliferation of leukemic cells Abstract In vitro experiments of this study was to explore human soluble vascular endothelial growth factor receptor 1 (sFLT  1) on leukemia cell proliferation in vivo. By RT  PCR detection of leukemia cell lines K562, HL  60, U937, and bone marrow LTC  IC VEGF mRNA, VEGF  R1 (FLT  1) mRNA expression detected by flow cytometry using the cell VEGF and VEGF  R1 (FLT  1) expression, ELISA assay of cell culture supernatant levels of VEGF, will sFLT  1 accession K562, HL  60 leukemia cell lines and bone marrow LTC  IC culture system, the application of MTT method to calculate the rate of cell growth inhibition. The results showed that: leukemia cell lines K562, HL  60, U937, and bone marrow LTC  IC are expressed VEGF, to the high volume of K562 expression of VEGF. K562 and HL  60 cell lines also expressed the FLT  1, U937 cells and bone marrow LTC  IC expression of FLT  1 expression is very low. sFLT  1 significantly inhibited the leukemia cell lines K562 and HL  60 growth, and with the increasing concentration of sFLT  1 and its inhibition rate increased to 48 hours after the drug inhibited the most obvious. Conclusion: sFLT  1 can inhibit the growth of certain leukemia cell lines, its inhibitory effects with the drug concentration increased, while the sFLT  1 in normal bone marrow cells had no effect. Keywords: VEGF FLT  1 leukemia cells Inhibition of Leukemic Cell Proliferation by Human Soluble VEGF  R1 Abstract The current study was purposed to investigate the inhibitory effect of human soluble vascular endothelial growth factor  1 (sFLT  1) on the proliferation of leukemic cells in vitro. Reverse transcription  polymerase chain reaction (RT  PCR) was used to detect the VEGF mRNA and VEGF  R1 (FLT  1) mRNA in K562, HL60, U937 leukemic cell lines and bone marrow LTC  IC. Flow c