Human trabecular organization and in vitro cultured human trabecular meshwork cells SPARC mRNA and protein expression of.docVIP

 PAGE \* MERGEFORMAT 11 Human trabecular organization and in vitro cultured human trabecular meshwork cells SPARC mRNA and protein expression of [Keywords:] of the human eye Expression of SPARC mRNA and SPARC protein in human trabecular meshwork and cultured human trabecular meshwork cells in vitro [Abstract] AIM: To investigate whether secreted protein acidic rich in cysteine (SPARC) and SPARC mRNA are expressed in human trabecular meshwork (TM) and cultured human trabecular meshwork cells (TMCs) and to explore the roles of SPARC protein in the pathogenesis of primary openangle glaucoma (POAG). METHODS: RTPCR and WesternBlot were used to detect SPARC mRNA and SPARC protein and immunofluorescence staining of cultured TMCs was performed by antiSPARC antibody. RESULTS: RTPCR of TM and TMCs using SPARC specific primers of the RNA displayed the presence of the predicted band of approximately 300 bp. WesternBlot showed a protein band of 43 Ku. CONCLUSION: Human TM and TMCs can express SPARC mRNA and its correlative protein, which influences the accumulation and degradation of extracellular matrix. [Keywords] trabecular meshwork; trabecular meshwork cells; secreted protein acidic rich in cysteine; extracellular matrix; primary openangle glaucoma [Abstract] Objective: To study the human trabecular meshwork Organization (TM) and in vitro cultured human trabecular meshwork cells (TMCs) whether it can express the secreted protein acidic and rich in cysteine (SPARC) mRNA and protein SPARC. Of SPARC protein in the original -onset open-angle glaucoma (POAG) in the pathogenesis. Methods: RTPCR and WesternBlot methods were used for trabecular tissues and cells were detected using anti-SPARC protein, immunofluorescence staining of cells staining. Results: Organization and cell RTPCR were 300 bp occurred at the expected electrophoretic bands, tissues and cells WesternBlot at Mr 43 * 103 protein bands appeared. TMCs anti-SPARC immunofluorescent staining showed cytoplasmic u