Human umbilical cord blood mesenchymal stem cells the general characteristics of its multi-differentiation potential of.docVIP

 PAGE \* MERGEFORMAT 22 Human umbilical cord blood mesenchymal stem cells the general characteristics of its multi-differentiation potential of Author: Wong Jing, Li Zhizhong, Xu Mao, late Chinese 【Abstract】 【Objective】 To observe the human umbilical cord blood mesenchymal stem cell proliferation, cell surface markers and multi-differentiation potential. 【Methods】 isolated from human umbilical cord blood mononuclear adherent cells were cultured in vitro. The cells were labeled monoclonal antibodies were detected by flow cytometry. Added to the cultured cells into bone, cartilage and into the fat into the inducer, test their multi-differentiation capacity. 【Results】 The umbilical cord blood mesenchymal stem cells in primary culture time was 7 weeks. Flow cytometry analysis showed that these cells CD29, CD13, CD44 and so on are expressed as positive, but they did not express hematopoietic stem cell surface markers. Join bone inducing agent will lead to the expression of alkaline phosphatase. Micro-ball culture method and join the cartilage inducing agents on the cells induced by Alcian blue staining showed that expression of proteoglycan. Prior to joining adipogenic inducers, it will lead to cell morphological changes, and the oil red O staining was positive. 【Conclusion】 through the body Gaiden method can be cultured from human umbilical cord blood mononuclear adherent cells obtained purified mesenchymal stem cells, umbilical cord blood mesenchymal stem cells is expected to become the alternative sources. Keywords: cord blood; mesenchymal stem cells; differentiation Umbilical cord blood is rich in hematopoietic stem cells has been well known, and the cord blood stem cell transplantation in clinical application of more than 10 years [1], and has become, after the bone marrow following hematopoietic stem cell transplantation is another important source. As for whether the umbilical cord blood contains mesenchymal stem cells (mesenchymal s