Human urinary tract epithelial cancer vaccines A preliminary study.docVIP

 PAGE \* MERGEFORMAT 14 Human urinary tract epithelial cancer vaccines A preliminary study [Abstract] Objective To screen phage random 12-peptide library was MUC1 carbohydrate antigen mimotope. Methods used to obtain the Ma695 purified antibody screening phage random 12-peptide library, by sandwich ELISA analysis of phage clones were determined positive clones were homologous DNA sequences and amino acid analysis, competitive inhibition experiments identified phage clones. Results After 3 rounds of screening, obtained 14 positive clones, DNA sequence analysis and deduced amino acid sequence: KHYDPFHHRMPQ, QADTARSVALAG, VPSKPDLHVRSI, MTPIHYWNHNRV. Identification results show that the four clones of phage display peptide inhibition rate of over 50%. Conclusion The sequence KHYDPFHHRMPQ, QADTARSVALAG, VPSKPDLHVRSI, and MTPIHYWNHNRV simulated MUC1 epitope. [Keywords:] MUC1 antigen mimotope phage random 12-peptide library A study on epithelium tumor vaccine in urologic syste Abstract ObjectiveTo identify and characterize the mimic epitope of MUC1 from phage display random 12 peptide library. Methods The purified Ma695 antibody was used to screen in phage display random 12 peptide library. The positive clones were identified by sandwich ELISA, competitive inhibition assay. The homologous comparison of amino acid sequences of positive clones was conducted through searching the protein sequence databank. The digestion analyses of amino acid sequences were also conducted by software. Results 14 positive clones were acquired after 3 rounds of screening. Amino acid sequences deduced from DNA sequences showed four different sequences as KHYDPFHHRMPQ, QADTARSVALAG, VPSKPDLHVRSI, MTPIHYWNHNRV. The inhibitory assay showed that the 4 mimic epitope peptides displaying on the phage surface could effectively inhibit the combination of antibody with antigen and the inhibitory rates of each mimic epitope were 50% higher than that in the control. Conclusion The results shows t