Human umbilical vein perfusion fluid mesenchymal stem cells isolation and identification of.docVIP

 PAGE \* MERGEFORMAT 4 Human umbilical vein perfusion fluid mesenchymal stem cells isolation and identification of Authors: LIU Xiaodan, Liu Bing, Li Xiushen, Mao Ning [Abstract] This study was designed to explore the human umbilical vein perfusion fluid the existence of mesenchymal stem cells and their isolation and amplification. After delivery from normal maternal perfusion liquid separation of the umbilical cord vein mesenchymal stem cells to observe cell morphology and flow cytometry analysis of their cell phenotype and cell cycle in vitro induction of osteogenesis, respectively, into the fat and cartilage cell differentiation. The results showed that: the source of umbilical vein perfusion fluid mesenchymal stem cells are fibroblast-like, high expression of CD29, HLA  ABC, CD166, CD105, CD73, CD44; did not express hematopoietic and endothelial mark (CD34, CD45, CD144, and CD14) . They may apply to the bone, fat and cartilage cell differentiation, consistent with mesenchymal stem cells, the basic functional characteristics. Conclusion: The existence of human umbilical vein perfusion fluid mesenchymal stem cells can be cultured and expanded in vitro can be used as cell therapy and tissue engineering in the future of the seed cells. [Keywords:] umbilical vein perfusion fluid Isolation and Identification of Mesenchymal Stem Cells from Perfusion of Human Umbilical Cord Vein Abstract This study was aimed to investigate whether mesenchymal stem cells (MSCs) existed in human umbilical cord vein and to establish the methods of isolation and expansion in vitro. The MSCs direved for perfusion of umbilical cord vein (UVMSCs) were collected after parturition of Healthy pregnant women. The morphology of MSCs and their differentiation potential into osteoblast, adipocyte and chondrocyte were observed the phenotype and cell cycle of MSCs were determined by using flow cytometry. The result showed that the mesenchymal stem cells separated from umbilical cord vein