Human umbilical cord mesenchymal stem cells culture and differentiation of osteogenic adipogenic Experimental Study.docVIP

 PAGE \* MERGEFORMAT 17 Human umbilical cord mesenchymal stem cells culture and differentiation of osteogenic adipogenic Experimental Study Of: EXAMINATION, Li Zhizhong, Wang Jing, Lin Yong new loud, Wu Bo Wen, Jiaogen Long, Shao Jianli [Abstract] Objective To investigate and optimize the human umbilical cord mesenchymal stem cells (hUCMSCs) and cultured in vitro proliferation of access methods and identified, induced to differentiate into bone cells and fat cell differentiation, the basis for its further application. Methods isolated by enzyme digestion Training hUCMSCs, by subculture, amplified, inverted optical microscope and atomic force microscopy cell morphology was observed, MTT cell proliferation assay curve, flow cytometry immunophenotype, in vitro to differentiate into bone, fat, the direction of induced differentiation, and through specific differentiation ability of staining. The results with enzyme digestion can Purification hUCMSCs. inoculated 24h, adherent cells were mostly long spindle shape, polygonal or fibroblast-like morphology, uniform size. P3, P7 growth curve of cells basically the same, proliferation ability. flow cytometry, 3 cells strongly expressed CD29, CD44, CD105, did not express CD34, CD45 and HLA DR. The osteogenic differentiation after 4 weeks showed strong alkaline phosphatase staining positive alizarin red staining showed significant calcium nodules, after 3 weeks of adipogenic induction, oil red O staining was positive, with a clear lipid droplets appear. Conclusion The establishment of a cultured and amplified in vitro stability of the method hUCMSCs. The single components in cultured cells, the rapid expansion, biological shape stability, and that they be induced to differentiate into bone cells and fat cell differentiation, tissue engineering is expected to become an ideal seed cells. [Keywords:] umbilical cord mesenchymal stem cells, differentiation, osteoblasts, adipocytes ABSTRACT: Objective To establish an