Human umbilical cord mesenchymal stem cells in culture and osteogenic differentiation of adipogenic.docVIP

 PAGE \* MERGEFORMAT 12 Human umbilical cord mesenchymal stem cells in culture and osteogenic differentiation of adipogenic Of: Zhao Lei, Wei-Yen Wang Add Fuchang Hao Wei Anhui group [Abstract] Objective To establish a small gestational age of human umbilical cord mesenchymal stem cells (hUCMSCs) Isolation and cultivation methods of hUCMSCs of adipogenic and osteogenic differentiation potential. Methods type collagenase and hyaluronidase digestion method from gestational age 12 ~ 18 w of fetal umbilical cord separation hUCMSCs; flow cytometry immunophenotype; apply different factors to fat cells and induced hUCMSCs osteoblastic differentiation and identification. hUCMSCs vitro results of fusiform cells uniform shape; expression CD29, CD44, CD73, CD105, CD166; did not express CD34, CD45, CD40, CD40L, CD80, CD86, HLA DR; Oil Red O, alizarin red staining and confirmed hUCMSCs RT PCR can differentiate into fat cells and osteoblasts. Conclusion A method for isolation and culture of small gestational age hUCMSCs, demonstrate its ability to adipogenic, osteogenic differentiation potential, is expected to be more tissue engineering cell therapy and an ideal seed cells. [Keywords:] umbilical cord mesenchymal stem cells; immunophenotype; fat cells; osteoblasts Mesenchymal stem cells (mesenchymal stem cells, MSC) mainly from adult bone marrow, compared with adult bone marrow MSC, the MSC from the fetal period with more powerful amplification capacity, low immunogenicity and a variety of sources, and many other advantages. who reported current umbilical cord stem cells (hUCMSCs) produced more than full-term fetus taken from the umbilical cord, and lower gestational age from abortion to separate the hUCMSCs umbilical cord has not been reported. the experiments were isolated from umbilical cord hUCMSCs , a preliminary study of its morphology, immunophenotype, and differentiation potential of the basic biological properties. 1 Materials and methods 1.1 Mate