Huang Xiaoyan Tablets pairs of quality standards.docVIP

 PAGE \* MERGEFORMAT 3 Huang Xiaoyan Tablets pairs of quality standards Author: Liu Penghan, Lu Lai-Xiang, Wei Geng-Long, Huang Long, Ho Sau right [Keywords:] double yellow anti-inflammatory tablets;,, baicalin;,, berberine;,, thin-layer chromatography;,, high-performance liquid chromatography Abstract: Objective To establish a double yellow anti-inflammatory tablets quality standards. Official Methods of thin-layer chromatography identification of agents of baicalin; high-performance liquid chromatography preparation of baicalin and berberine hydrochloride. High-performance liquid chromatography conditions Vp-ODSC18 column (4.6 mm * 150 mm, 5 μ m), mobile phase of acetonitrile: 0.02 mol / L potassium dihydrogen phosphate (24:76); a flow rate of 0.8 ml / min; detection wavelength 270 nm; column temperature: 30 ℃ . The results of TLC spots were clear and easy identification; high-performance liquid chromatography for determination of baicalin and berberine in 5 ~ 50 μ g / ml, and 2.04 ~ 20.4 μ g / ml Jun Cheng a good linear relationship within the average recoveries were of 102.97%, RSD 0.81% and 99.68%, RSD 0.82%. Conclusion The method is simple, reproducible, and can be used to double yellow anti-inflammatory tablets quality control. Keywords:: double-yellow anti-inflammatory tablets; baicalin; berberine hydrochloride; thin-layer chromatography; high-performance liquid chromatography Quality Standard for Shuanghuangxiaoyan Tablet Abstract: ObjectiveTo establish the quality standard for Shuanghuangxiaoyan Tablet. MethodsUsing TLC to identify baicalin and HPLC to determine the contents of baicalin and berberine hydrochloride in Shuanghuangxiaoyan tablet. The HPLC system consisted of Vp-ODS C18 column (4.6 mm * 150 mm, 5 μ m ), acetonitrile: 0.02mol / L KH2PO4 (24:76) mixtures as mobile phase, detection wavelength at 270 nm, flow rate of 0.8ml/min, column temperature: 30 ℃ . ResultsThe spots in the TLC were clear and distinguishable, the linear ranges of ba