Hyperlipidemia in experimental nephrotic rat kidney TGF-β 1 gene expression in.docVIP

 PAGE \* MERGEFORMAT 15 Hyperlipidemia in experimental nephrotic rat kidney TGF-β 1 gene expression in [Abstract] Objective This study was high-fat diet on adriamycin nephropathy (ADR) rat model of renal TGF-β 1 gene expression, and to explore the Simvastatin and benazepril on the ADR rat kidney TGF-β 1 gene expression in impact. Methods According to the sequence of rat TGF-β 1cDNA designed TGF-β 1 reverse transcription-polymerase chain reaction (RT-PCR) primers for RT-PCR method in order to observe the different groups of rat renal TGF-β 1mRNA at 4,8,12 weeks expression, and to GAPDH as an internal reference; tags (digoxin) TGF-β 1 probe, in different groups of rats on renal tissue frozen sections for in situ hybridization to detect rat kidney at 12 weeks the expression of TGF-β 1mRNA situation. The results of high-fat diet rats 4,8,12 weeks after ADR renal expression of TGF-β 1mRNA gradually upward, 8,12 weeks was significantly higher than normal feed nephropathy in rats, 12 weeks in situ hybridization results showed that the high-fat diet nephropathy renal TGF-β 1mRNA enhanced significantly in order to tubulointerstitial mainly ordinary feed nephropathy TGF-β 1mRNA expression mainly in the tubulointerstitium, showing focal distribution; simvastatin group and benazepril on rats a high renal expression of TGF-β 1mRNA fat diet group was significantly lower in rat kidney; normal rats high-fat diet group, expression of TGF-β 1mRNA higher than normal at 12 weeks the control group. Conclusion High-fat diet and promoting renal fibrosis in rats with ADR factor TGF-β 1mRNA expression was significantly increase with time expressed in tubulointerstitium of the main; Simvastatin and benazepril significantly reduced the expression of renal TGF-β 1mRNA, Xin valve Statins reduce the role of renal TGF-β 1mRNA mechanism may reduce the hyperlipidemia associated with the activity of the RAS, but also may indirectly inhibit the role of the MVA pathway; and benazepril on rats wi