Improved three-cuff technique for orthotopic small bowel transplantation model in rats.docVIP

 PAGE \* MERGEFORMAT 14 Improved three-cuff technique for orthotopic small bowel transplantation model in rats Of: Xiu Yu, Xiang-Ming, Fan Lin, He Jien, Linzi Jing, Zhao Wei [Abstract] Objective To improve the vascular reconstruction, simplify surgical procedures, the establishment of a stable model of orthotopic intestinal transplantation in rats. Methods of vascular reconstruction of three-cuff line: superior mesenteric artery was carried by the donor and recipient abdominal aorta abdominal aortic segments Cuff sleeve from the cuff anastomosis, the donor portal vein and recipient left renal vein anastomosis by the cuff Cuff sleeves. Results A total of 30 patients were transplanted successfully in 27 cases, 90% success rate. for body average operation time-consuming (42 + -5) min, time-consuming for intestinal repair (3 + -1) min, recipient operation time-consuming (65 + -10) min, warm ischemia 0min, cold ischemia (32 + -3) min, three sets of tubes were time-consuming (10 + -1) min, the total operation time (160 + -10) min. Conclusions and Application of strict protection for the three-cuff method of intestinal reconstruction of blood vessels, helps to quickly establish a simple and stable, high success rate fewer complications of segmental orthotopic small bowel transplantation model in rats. [Keywords:] rat; small bowel transplantation; in situ; vascular reconstruction ABSTRACT: Objective To improve the vascular reconstruction technique, simplify the surgery process and establish a stable animal model for small bowel transplantation (SBT) in rats. Methods Reconstruction of vessels with tri cuff technique: Abdominal aorta of the donor attached to the graft was anastomosed to the abdominal aorta of the recipient with two Cuffs separately; the hepatic portal vein of the donor attached to the graft was anastomosed to the left renal vein with a Cuff. Results There were 30 cases of transplantation, 27 of which succeeded with a success rate of 90.0%. Th