Inflammatory factors in uremic rat brain ischemia-reperfusion injury.docVIP

 PAGE \* MERGEFORMAT 13 Inflammatory factors in uremic rat brain ischemia-reperfusion injury OF: Juan Chen Xiao Feng Sun Ye Min Dan Hook [Abstract] Objective To investigate the uremic rats with cerebral ischemia and reperfusion (I / R) Dynamic changes of the inflammatory factor. Methods healthy male Wistar rats of clean grade 30, were randomly divided into three groups of uremia (CRF group) 6 uremia with cerebral I / R group (CRF + I / R group) 24, prepared uremia model, into a mold, then take the same 30 rats were randomly divided into control group 6, the simple brain I / R group of 24. to I / R group and the CRF + I / R group then middle cerebral artery occlusion, 2 h after ischemia reperfusion, according to different time points after reperfusion (0,2,12, 24 h) I / R group, CRF + I / R group was divided into 4 subgroups, each subgroup 6. I / R group and CRF + I / R group at time points after reperfusion drawn normal feeding in the control group harvested after 3 ~ 7 d, CRF group after modeling 0 ~ 3 d derived, RT PCR assay in brain tissue TNF @, IL 1, IL 6 mRNA expression. Results TNF @ mRNA: the control group CRF rats with brain tissue TNF @ mRNA expression was not significant (Pgt; 0.05), CRF rats compared with TNF @ mRNA expression in I / R group at each time point lower (P lt;0.05); cerebral I / R two groups of rats with TNF @ mRNA expression in the 2 h time point the most, then decreased gradually; CRF + I / R group at each time point than TNF @ mRNA expression of CRF group, I / R group increased (P lt; 0.05). IL 1 mRNA, IL 6 mRNA: CRF rats, IL 1 mRNA, IL 6 mRNA expression increased compared with normal control group, but the difference was not significant (Pgt; 0.05); and I / R group at each time point compared, CRF rats IL 1 mRNA, IL 6 mRNA expression were significantly lower (P lt;0.05); cerebral I / R group rat brain IL 1, IL 6 mRNA expression gradually with reperfusion time increased and peaked between 24 h; and I / R group and the CRF group