Inner Mongolia Han molecular genetics of coronary heart disease patients.docVIP

 PAGE \* MERGEFORMAT 8 Inner Mongolia Han molecular genetics of coronary heart disease patients [Abstract] Objective To investigate the Inner Mongolia region of the ACE gene in patients with coronary heart disease Han I / N polymorphism, healthy individuals with the Han comparison, analysis of their genetic characteristics. Methods 54 patients with coronary heart disease in patients with ACE gene intron DNA application more than 16 state site flanking the precursor for PCR amplification. Allele on agar gel electrophoresis, ethidium bromide staining, UV-lamp observation. The results in patients with DD, DI and Ⅱ genotype frequency is 0.57, 0.18 and 0.24, Han healthy population of the DD, DI and Ⅱ genotype frequency is 0.33, 0.31 and 0.35. Coronary heart disease in patients with DD genotype and allele frequencies were significantly higher DD. Conclusion In the northern Chinese Han population study found that ACE gene I / N polymorphism is associated with coronary heart disease. [Keywords:] ACE gene; polymorphism; coronary heart disease [Abstract] Objective To investigate the insertion / deletion (I / N) polymorphism of angiotensin converting enzyme (ACE) with CHD in Han, and analysis their genetics characteristics. Methods 34 patients genomic DNA was amplified by polymorphic region in intron 16 of the ACE gene. Allele were detected on agarose gel stained with ethidium bromide.Results The frequencies of DD, DI and II genotype were 0.57, 0.18 and 0.24in CHD; and 0.33, 0.31 and 0.35 in the control respectively. Conclusion The polymorphism of ACE gene are associated with CHD in Hans. [Keywords:] angiotensin-converting enzyme gene; polymorphism; CHD Coronary heart disease is a multi-gene disease, and its location and its related gene research has become quite a long period of time and in the future the focus of concern and difficulty. Guangdong, one study shows that to carry angiotensin-converting enzyme (ACE) gene DD-type increased risk of coronary heart disea