Integrated Optimization of multi-index solvent extraction conditions Pueraria lobata.docVIP

 PAGE \* MERGEFORMAT 11 Integrated Optimization of multi-index solvent extraction conditions Pueraria lobata Authors: Song Tong, Lu Li Qiang, Zhou Yunyun, Peng Chao, Xing Ying, Zhi-Yong Tang [Abstract] Objective To establish the best conditions for solvent extraction Pueraria. Methods using high performance liquid chromatography and atomic fluorescence spectrometry determination of puerarin in Pueraria Extract and lead and mercury content of the element in order to puerarin and lead and mercury content of the element evaluation index, which affect solvent extraction and Pueraria active ingredients conditions were optimized. The results of 80% ethanol solvent; temperature of 100 ℃ ; solvent volume of 35 ml; extract time and frequency is 3. Conclusion The extraction method so that units of the active ingredient Pueraria lobata of lead, mercury content greatly reduced, and in accordance with the restrictions imposed by the national standard requirements. [Keywords:] puerarin; puerarin; lead; mercury; solvent extraction Abstract: ObjectiveTo establish the optimum solvent extraction concentrations of Pueraria lobata. Methods The conditions of solvent extracting active ingredient from pueraria lobata were optimized, and evaluating indIcators were contents of puerarin, lead and mercury, which were determined by high performance liquid chromatography and atomic fluorescence spectrometry. ResultsThe optimum condition was extracting pueraria lobata three times for three hours with 35 ml 80% ethanol as solvent at 100 ℃ . ConclusionContents of lead and mercury in active ingredient reduced greatly, and in line with limited demand of national standard. Keywords:: Pueraria lobata; Puerarin; Lead; Mercury; Solvent extraction Kudzu is a leguminous plant Pueraria lobata, or kudzu roots, there are mountain ginseng’s good name, the main active ingredient of puerarin, daidzin, daidzein and other isoflavones. Kudzu is commonly used traditional Chinese medicine, with fever Jie