Intraperitoneal injection of adenosine in rat hippocampus the expression of cfos.docVIP

 PAGE \* MERGEFORMAT 10 Intraperitoneal injection of adenosine in rat hippocampus the expression of cfos Study: Song Ying Fu Libo Wang Xuebin Liu Fenglian [Abstract] Objective To study the intraperitoneal injection of adenosine in rat hippocampus c fos protein expression. Methods and their intra-abdominal injection of non-specific adenosine receptor antagonist theophylline, the use of Fos immunohistochemistry (ABC method) to observe the c fos protein in the hippocampus region of the distribution. Results rat hippocampus after injection of adenosine in the c fos protein expression was significantly increased compared with normal saline, injected into rat hippocampus after theophylline the c fos protein expression control group, no significant change. Conclusion hippocampus adenosine can promote the expression of c fos, adenosine in the hippocampus region in regulating sleep may have an important role. [Keywords:] adenosine and hippocampus, c fos, immunohistochemistry staining Adenosine (Adenoisine, Ado as the central nervous system (CNS a neuromodulator, can reduce the excitability of CNS neurons in many parts of central inhibited (1,2). Ado in the CNS, directly or indirectly involved in sleep , sedative and analgesic effects, is an important factor in sleep regulation. In ischemia, hypoxia and the absence of sufficient blood perfusion, the brain concentration of Ado in the rapidly rising increase in endogenous Ado and energy deficiency on the metabolism of tissue injury caused by a protective effect on sleep has a facilitating role. a lot of sleep factors, such as interleukin (IL 1, melatonin, etc., by making the c fos protein in CNS and c fos mRNA expression levels increased to promote sleep (3) . hippocampal area is an important central part, c fos gene in the hippocampus could be slightly stimulated expression. the present study, intraperitoneal injection of Ado and immunohistochemistry to observe the Fos protein distribution in the hippocampus,