Isoflurane reduction in isolated rat liver hypoxia - reoxygenation injury and Kupffer cells in the.docVIP

 PAGE \* MERGEFORMAT 8 Isoflurane reduction in isolated rat liver hypoxia - reoxygenation injury and Kupffer cells in the Author; Li Quan, Yu Wei-feng, Cao Yunfei, Li-Qun Yang, Xin Lu, Zhou Tao pulse 【Abstract】 Objective To reduce isoflurane in isolated rat liver hypoxia - reoxygenation injury occurs mainly at what stage, as well as the relationship between Kupffer cells. Methods The rat liver removed and placed in isolated rat liver perfusion instrument in order to by 95% O2 / 5% CO2 saturation improved g - Lin bicarbonate buffer constant pressure perfusion (aerobic condition with 1.2kPa, hypoxia with 0.2kPa), the maintenance of physiological pH value and temperature, real-time monitoring of changes in perfusion fluid LDH. 2MAC isoflurane were used for hypoxia period, reoxygenation period and hypoxia - reoxygenation period. With GdCl3 selectively inhibit Kupffer cell activity. Results (1) 2MAC isoflurane for anoxia - reoxygenation period and the pure oxygen used in recovery phase could inhibit LDH elevated, but simply had no significant effect for the period of hypoxia. (2) 2MAC isoflurane and (or) GdCl3 group can inhibit the liver hypoxia - reoxygenation LDH increase. Conclusion Isoflurane Protection anoxia - reoxygenation main function of the liver from injury to play in the reoxygenation period, isoflurane during reoxygenation period to play a protective effect may be related to inhibition of oxygen free radicals caused by reoxygenation eruptions, its role and dry No cell activity closely related. Keywords: Isoflurane; isolated rat liver; hypoxia - reoxygenation injury; Kupffer cells 【Abstract】 Objective To investigate which stage is the most important and the relationship with kupffer cell, during isoflurane decrease anoxia-reoxygenation injury in isolated rat liver.Methods We established the model of isolated rat liver.It included artificial lung, pump, pressure meter , flow meter, thermostat, humidifier and monitors.Rat liv