Isolation and culture of MSCs in peripheral blood of GCSF mobilization response.docVIP

 PAGE \* MERGEFORMAT 11 Isolation and culture of MSCs in peripheral blood of GCSF mobilization response Of: Tang Jun, Zou Zhongmin, Chun-Lei Zhang, Wang Junping, Ai Guoping, Su Yong-ping [Abstract] Objective To establish a multi-variety case of peripheral blood stem cells (MSCs isolation and culture of method of its granulocyte colony stimulating factor (G CSF mobilization responses. Approach using percoll (1.131 g / mL of peripheral blood separation is more than all the mononuclear cells were adherent cultured MSCs. with G CSF as a mobilizing agent, bone marrow and peripheral blood derived MSCs, before and after mobilization of fibroblasts counting colony forming units (CFU F. The results of these are more isolated MSCs in peripheral blood culture, the success rate are different. In the experimental conditions, can be isolated and cultured stable direct the MSCs.G CSF mobilized peripheral blood, the bone marrow-derived MSCs, the number of CFU F was significantly higher (P lt;0.01, peripheral blood source of MSCs, the number of CFU F was significantly higher (P lt;0.01, nearly 4 times. Conclusion of different species were isolated and cultured MSCs in peripheral blood of different difficulty, because of the direct physiological conditions that exist in peripheral blood of MSCs led to less The. G CSF can effectively mobilize the bone marrow and peripheral blood MSCs. [Keywords:] mesenchymal stem cells, mobilization, granulocyte colony stimulating factor, cell culture Abstract: Objective To establish the isolating cultural method of MSCs from some genus peripheral blood (PB), and explore the mobilization response of MSCs by G CSF. Methods MSCs from some genus PB were isolated by percoll (1.131 g / mL) and carried out adherent culture. MSCs derived bone marrow and PB were cultured and its CFU F were counted after mobilization by G CSF. Results PB MSCs were isolated and cultureed successfully or unsuccessfully from different genus. Under our experiment