Isoliquiritigenin on Hela cell redox state of.docVIP

 PAGE \* MERGEFORMAT 15 Isoliquiritigenin on Hela cell redox state of Of: Chen Peng, Juan, Wang Zhenhua, Qiu-Sheng Zheng [Abstract] Objective To investigate the in vitro antioxidant isoliquiritigenin Hela cells and its effect on the impact of redox state were investigated isoliquiritigenin Hela cervical cancer cell proliferation and inhibition of reactive oxygen species. Methods determined by chemical methods for hydroxyl isoliquiritigenin base, DPPH, superoxide anion scavenging rate and the inhibition of lipid peroxidation inhibition, investigated the antioxidant effect; SRB cell viability was determined to intracellular ROS fluorescent probe DCFH-DA, the fluorescence intensity measured to characterize the intracellular ROS level kit, SOD and CAT assayed the vitality of its Effect on the intracellular redox state. The results isoliquiritigenin has strong antioxidant effects, and dose dependently inhibited the proliferation of Hela cells; with different Glycyrrhizin concentration showed 1 ~ 7.5 mg / L anti-oxidation, higher than 7.5 mg / L concentration of prooxidant changes. Conclusions isoliquiritigenin concentration dependently inhibited the proliferation of cervical cancer Hela cells; isoliquiritigenin clear cells with low concentrations reactive oxygen species and high concentrations promote the formation of free radicals, and promote apoptosis of Hela cells. [Keywords:] isoliquiritigenin; antioxidant; free radicals; reactive oxygen species Abstract: ObjectiveTo investigate the influence of isoliquiritigenin (ISL) on the oxidative-reductive state in Hela cells and to study the relationship between the intracellular reactive oxygen species (ROS) and the inhibitory effects on Hela cell proliferation induced by ISL. MethodsThe SRB method was used to determine the effect of ISL on the scavenging rate of hydroxyl radical, DPPH, superoxide anion and on the inhibition rate of lipid peroxidation. The intracellular ROS levels was measured with the fluor