Klotho protein on the C57BL - 6 mice the number of substantia nigra neurons of.docVIP

 PAGE \* MERGEFORMAT 13 Klotho protein on the C57BL / 6 mice the number of substantia nigra neurons of Study: Core of Du Hongxia Fang Zhang Zhan Li Xinhua Mi Man [Abstract] Objective To observe the Klotho protein on the C57BL / 6 mice the number of substantia nigra neurons and to explore its possible mechanism. Methods C57BL / 6 mice were divided into 3 groups: wild-type mice (WT, Klotho gene over-expression of type ( Tg mice, Klotho gene knockout mice (KO of the six, behavioral observation and weight measurement, using tyrosine hydroxylase (TH) immunohistochemistry observed changes in substantia nigra neurons. Results KO mice and WT , Tg mice was significantly lower than body weight (P lt;0.01.WT mice compared with Tg mice had no significant difference in body weight (Pgt; 0.05, compared with WT mice KO mice substantia nigra neurons Tg increases in the number of substantia nigra of mice reduced the number of neurons among the three groups were significantly different (P lt;0.01. Conclusion Klotho gene may affect the nigrostriatal dopaminergic neurons within the number. and WT mice KO mice compared with the substantia nigra of the original Tg increases in the number of substantia nigra neurons in mice reduced the number may be Klotho FGF23 co-receptor negative feedback mechanism of regulation. [Keywords:] Klotho protein, dopaminergic neurons in the substantia nigra Klotho protein is closely related to human aging (1), the protein deficient mice with human aging may appear similar to a variety of behavioral and pathological changes: shortened life expectancy, movement disorders, sexual dysfunction, dementia, cataracts, atherosclerosis, osteoporosis, such as emphysema and immune function, gene knockout mice abnormal gait, step length was significantly shorter on average, similar to human Parkinson’s disease (PD patients with gait, the use of certain methods of mice to regain genes, can alleviate these symptoms (2). researchers used gene technology bre