Lamotrigine on perinatal hypoxic seizures result in neuroprotection in neonatal rats.docVIP

 PAGE \* MERGEFORMAT 17 Lamotrigine on perinatal hypoxic seizures result in neuroprotection in neonatal rats Study: Huan Wang Ming, Deng Yan, JIANG Li, He Nongyue [Abstract] Objective: To investigate the lamotrigine (LTG) on perinatal hypoxic neonatal rats led to seizures (after birth 10 d) the neuroprotective effect. Methods: Newborn rats were randomly divided into saline group hypoxia, lack of oxygen LTG group, saline group and normoxia normoxia LTG group 4. hypoxic saline group (n = 26): Method for producing oxygen using gradient model of acute hypoxia (oxygen concentrations of less than 4%), lack of oxygen before has been normal saline 0.5 h; hypoxia LTG group (n = 26): intraperitoneal injection of LTG (20 mg kg-1), 0.5 h then placed above the oxygen devices; normoxic saline group (n = 26 ): intraperitoneal injection of saline into the normal oxygen concentration in the same device; normal oxygen LTG group (n = 26): LTG after intraperitoneal injection into the device above the normal oxygen concentration. Nissl assessed 1 h after the seizure and Health 30 d after cell loss in rats situation, Timm staining 30 d after birth, mossy fiber sprouting in rats with the situation, pentylenetetrazole measured 30 d after birth, seizure susceptibility in rats. Results: Nissl staining showed that large groups mice 1 h in seizures and 30 d after birth, the two time points showed no significant loss of neurons. hypoxic LTG group of perinatal hypoxic seizures in the extent and sensitivity of hypoxic saline group with no significant difference normoxic groups showed no seizures. but after birth 30 d, hypoxia LTG group of pentylenetetrazol seizure threshold in the saline group was significantly higher than hypoxia (P lt;0.05), lower than the normoxic group (P lt;0.01), no significant difference between normoxic groups (Pgt; 0.05). hypoxic LTG group 30 d after birth, the dentate gyrus granule mossy fiber sprouting in the upper fraction was significantly lower than the