Large-scale protein mass spectrometry identification of peptides modified with N-terminal ring phenomenon.docVIP

 PAGE \* MERGEFORMAT 15 Large-scale protein mass spectrometry identification of peptides modified with N-terminal ring phenomenon Study: Chuang Zhao Liyan Zhang Yang Lu Cai Yun Yu-Lin Deng Yu-Kui Zhang QIAN Xiao Jun [Abstract] sample preparation for protein amino acid residues introduced into a phenomenon - N-terminal peptide of protein restriction modification phenomenon of ring A preliminary study showed that many of the glutamine (Q or ammonia half-acetylation cystine (CAM_C peptide residues will occur starting the ring of N-terminal modification, and the modification reaction is not complete, the samples modified in the same two states with non-modified frequently exist, and the ring of the modified peptides RP retention time delay. in the database retrieval add ring modification, can improve the success rate of protein identification. The result is a large-scale protein mass spectrometry data analysis provides a valuable reference. [Keywords:] N-terminal peptides, ring, scale, protein identification Abstract Biological mass spectrometry has been developed for the large scale protein identification. The successful identification of protein in proteomic study is based on an effective match of MS data to the sequence in database. Because of the diversity and heterogeneity of protein modification, the experimental data obtained by mass spectrometry does not match the theoretical value sometimes, which makes about 90 percent or more of the tandem mass spectra not be effectively identified. This has become one of the most important technique issues to be resolved in current proteome research. The N terminal cyclization of peptides, as one of a variety of modification introduced in sample preparation, has been preliminarily studied in this work. The result showed that N terminal cyclization occurred at the most of the glutamine (Q) or carbamoylmethyl cysteine #8203;#8203;(CAM_C) residues and the reaction is often incomplete or partial, both types of p