Laryngeal squamous cell carcinoma of the differences in gene expression of SPARC.docVIP

 PAGE \* MERGEFORMAT 2 Laryngeal squamous cell carcinoma of the differences in gene expression of SPARC Author: car Yiqun, Wang Hai, Shi Xuedong, Jun Qi, Guo Jian, Liu Zhi-hua [Abstract] Objective To investigate the cysteine-rich acidic protein (SPARC) in laryngeal squamous cell carcinoma proliferation, invasion and metastasis of the role and significance. Methods Semi-quantitative reverse transcription  polymerase chain reaction (RT  PCR) analysis of SPARC in different laryngeal squamous cell carcinoma and its adjacent normal mucosa, the amount of differential expression. Immunohistochemical study of the use of technology SPARC in laryngeal squamous cell carcinoma and its adjacent normal mucosa, the expression of differences in protein expression in the same time to locate. Results RT  PCR analysis of SPARC in 20 pairs of matched samples in the expression, in which 16 pairs of laryngeal squamous cell carcinoma was significantly higher than their corresponding normal mucosa, in the four pairs of differential expression is not obvious, R = 80.0% ( there are differences in the number of cases / total number of cases). Immunohistochemistry was analyzed 30 pairs of matched surgical specimens of laryngeal squamous cell carcinoma paraffin sections (tumor tissue and normal mucosa), of which 25 pairs of SPARC protein in the cancer nest cells than the adjacent normal mucosa, 5 cases in the nest cell carcinoma and adjacent normal mucosa tissue expression is not obvious, R = 83.3%. The same time, SPARC protein in the different degree of differentiation of laryngeal carcinoma and analysis of SPARC was found in poorly differentiated laryngeal carcinoma with well-differentiated carcinoma was no significant difference (P