Laser scanning confocal microscope in the study of the fluorescence intensity determination conditions.docVIP

 PAGE \* MERGEFORMAT 8 Laser scanning confocal microscope in the study of the fluorescence intensity determination conditions 【Abstract】 Objective In this paper, laser scanning confocal microscope for fluorescence intensity in terms of which were measured in detail. Select and set the laser scanning confocal microscope in all parameters, using indirect immunofluorescence assay of the modified surface of human gingival fibroblast secretion of extracellular matrix fibronectin FN and collagen type I conduct a quantitative fluorescence intensity . The results of cells after vaccination FN surface fluorescence staining intensity differences between the four groups of materials, no significant, indicating changes in surface chemical composition of the formation of the FN had no effect. But the four groups of materials on the I-collagen secretion were different. Conclusion select and set the appropriate laser scanning confocal microscope in the determination of fluorescence intensity conditions, it can reflect the real and effective results of the experiment. Keywords: laser scanning confocal microscope fluorescence intensity research on determine condition of fluorescence intensity in confocal laser scanning microscopy 【Abstract】 Objective To explore the conditions under fluorescence intensity is determined in confocal laser scanning microscopy.Methods The parameters of confocal laser scanning microscopy were set and the effects of these modifications on the response of the fibro-conglutination albumen and type I collage n of human gingival fibrob ˉ lasts on indirect immunityfluorescence were observed, and the fluorescence intensity of the effects was measured.Re ˉ sults After the cells grafted on the material surface, the fluorescence dye intensity of the fibro-conglutination albu ˉ men had no notable differ ence in materials of the four groups, the alter of the chemistry component of the material sur ˉ face had