Lasting stability in the Rat - mouse hematopoietic chimera model.docVIP

 PAGE \* MERGEFORMAT 7 Lasting stability in the Rat - mouse hematopoietic chimera model Authors: WU Xiao-Juan, Li Fu, TANG Xiang-Feng, Shi Lei [Keywords:] bone marrow transplant Establishment of stable rat-mouse hematopoietic chimera model [Abstract] AIM: To establish a stable animal model of hematopoietic chimerism. METHODS: Conditioned SD rats received bone marrow cells from BALB / c mouse to induce xenogeneic chimeric rats. Thirty days later, lethally irradiated BALB / c mice received bone marrow cells from chimeric rats and their survival rate was monitored daily. Recipients splenic cells were stimulated in mixed lymphocyte culture (MLC) with xenoantigen and skin transplantation was carried out on day 30. RESULTS: Acute graft versus host disease was significantly alleviated, the survival time was prolonged and T cell proliferation was inhibited. More stable xenogeneic chimerism over 90 d was achieved via reverse bone marrow transplantation. CONCLUSION: A longterm xenogeneic chimera model is established by transplanting bone marrow cells of chimeras. [Keywords] chimera model; wholebody irradiation; bone marrow transplantation [Abstract] Objective: To establish a lasting and stable hematopoietic chimera model. Methods: SD rats after pretreatment via the tail vein injection of BALB / c mouse bone marrow cells to induce the formation of chimeric-specific tolerance in SD rats. 30 d, then this reverse chimera of rat bone marrow transplant after lethal irradiation BALB / c mice to monitor the survival rate of mice, 30 d after transplanting to do skin graft and mixed lymphocyte culture, observing the small the rate of change in chimeric mice. Results: The chimeric mouse model with only mild graft-versus-host disease manifestations, survival time was significantly prolonged, more stable rate of post-transplant chimerism, prolonged survival of skin grafts, with a control group than to have a significant difference. Conclusion: The bone marrow chimerism by ent