Leech preparation and the quality of freeze-dried powder injection of.docVIP

 PAGE \* MERGEFORMAT 13 Leech preparation and the quality of freeze-dried powder injection of [Abstract] Objective To prepare freeze-dried powder injection leech and establish quality control methods. Screening method of the type and amount of excipients, the optimal prescription and preparation techniques, high performance liquid chromatography (HPLC determination of leech peptide (whitmanin content and investigated as the main indicators of quality of preparation. Another study its stability through long-term experiments. The results using 5% mannitol as excipient better, determination of the average recovery was 99.8% (RSD = 1.8% , left at room temperature about 12 months was stable. CONCLUSION The freeze-dried powder injection prescription leech reasonable process is feasible, the quality control. [Keywords:] leech, freeze-dried powder injection, leech peptide, preparation Study on the Preparation Technique and Quality of Mahuang Freeze-dried Sterile Injection Powder ZHONG Shan, YANG De po, CUI Zheng (School of Pharmacological Sciences, Sun Yat-sen University, Guangzhou 510080, China; School of Traditional Chinese Materia Medica, Shenyang Pharmacological University, Shenyang 110016, China Abstract: ObjectiveTo prepare Mahuang freeze-dried sterile injection powder and establish a quality control for this drug. MethodsThe best formulation and preparation technique of the powder for injection was optimized by screening the variety and quantity of whitmanin.The contents, which were determined by HPLC , were taken as the main indexes to study the quality. The stability was observed through test. ResultsTaking the 5% mannitol as excipient, the mean recovery result was 99.8% (RSD = 1.8%), and the preparation was stable after 12 months storage at room temperature.ConclusionThe formulation is reasonable, the preparation technique is feasible and the quality is controllable. Keywords:: Leech; Sterile injection powder; Whitmanin; Preparation techn