邻苯二甲酸丁苄酯BBP对雄性F1大鼠海马BDNF、CREST、Spinophilin mRNA表达的影响.pdfVIP

邻苯二甲酸丁苄酯(BBP)对雄性Fl大鼠海马 BDNF、CREST、Spinophilin mRNA表达的影响 庄梅珠李元锋李涛石年 (华中科技夫学醚济医学院。湖=l艺武汉430030) 摘要：翻的：研巍BBP对攘戮Fl火蔽瓣玛种缝行为鬏霄鞠蓑港嗣mRNA浚选的彩晌。方法：凝PNDl、7、21 雄潍仔躐的簿玛，撬取憝RNA《逆转泶袋键cDNA，崩装Ⅳ兜迩缀飚耩缓扩增，数l}-actin为蠹参梭浏海马e．硒s， C魁鎏T、BDNF、spinophilin m疑NA瀣达。鳓鬃：(t)PNDI壤憔仔鼠海玛肇闲mRNA袭遮敬爱2 CRESTmRNA 谯抵粼燧烈浚然下谰(，≮0。05》，凌选餐必簿躐缀的0．6翁，两谯；扎蕊刹熬缀瀣达。f二瓣(，》《O．05)，分躐为瓣黻 觊躺1．5僚承j I．6镶；spinoph[iin mRNA假程中潮镶维的滏达￡例(PO，05)，淘麓爨鬃瓣I．3铸。(2)PND7雄 性谨鼠港马繁闲mRNA滏遮渡变：C疑崴S彳、BDNF、spinophilin tuRNA浚逑程中、怒荆麓缀均”F调(P《0。05)。 CREST mRNA程}p、藤割鬃缀l袭逸麓分割为对照缀豹0．56德釉0．49德；BDNF mRNA猩；n簪、豳测瀚缀涎达麓 分壤为对篓鹱缀的0，润戆翻0．77协：spinophilinmRNA谯；轧离滩墩爨i裘逖壤瓣渤对照耀的0。36掺。(3)PND2I 雄然仔藏海马籍麓阏mRNA袈遮邋嘲懿潋饔《胁O．05)。弩麓照燃撼羧，翁鲣嫒维瓣憷仔斌备刚4闯点海马沓fos mRNA装运嚣溪疆渡变(艘田。05)。绐谂：BBP甜蘩参雄攮箨鼠(黔qDI、PND7)海锄蕊围(c鬏ES丁、BDNF、 spinophilin)mRNA淡迭钉一定的辫响；熟爆鬟质21天(PND2l》，鑫缝攥斑壤摊仔鼠海岛j兰辨麓隧l裘选蘩滚 楚照藩缆纯。 关键调；部笨二甲酸T苇黪{CREST；BDNF：spinophilin Effects of BBP on the Expression ofBDNF，CREST, Spinophilin mRNA in the Hippocampus of the Fl Male Rats ZH；ANG Meizhu,LI Yuanfeng，LI Tao，SH!Nian (Depa内nent of Health Toxicology，MOE Key Laboratory ofEnvironment arm Health，School of Public Health，Ton西i M醚ical College，Huazhong University of Science and Technology， Wuhan 430030，China) Abstract Objective：To investigate the effects of Butyl挺嘲I phthalate僻BP)on the tuRNA expressions of the genes in the hippocampus心iated tO neurobehavioral development in the male ofi吝pdng．Methods：The hippocampus ofthe male offspring were obtained at PND!，7，and 21。After abstracting the total RNA，cDNA was obtained from reverse transcription，Real time PCR waS 作者簿介：庄掺珠t蠹。磺：t黼究耋，点臻从廖琢境瓷激漆的卒喾缝霉避疆宽，E-msil：mcizhuzhuang@y涨oo。en。祗年，爨， 博：龟黪蛹-圭缓扶氍教努翻环境豢激索的静￡囊谶霉§硝就，E-mail：snian@mails．tjmu．础。雠． introduced to detect the expression Ievels of m酬A in genesof。-fos．CREST,BDNF and spinophilin。 Results：fI)Changes ofmRNA—expression ofgenes in hippocampus at PNDI：compared to the control group,the expression ofCREST mRNA was down—regulated fPO．05)in the low-dose group(0。6 fold) and up-regulated(p《O。05)in the mid-and high—dose gmups(1。5 and 1．6 fol由．The expression o