邻苯二甲酸二丁酯对雄性子代大鼠学习记忆及海马spinophilin表达的影响.pdfVIP

邻苯=甲酸=丁酯对雄性子代大鼠学习记忆及海马 spinophiIin表达的影晌 李元锋庄梅株李涛 石年 (华巾科技走学I浏浇医学院公藏止生学藏卫生毒理学羲武汉430030) 籀要}霪鼹埒目的}筏察邻笨。二二甲黢二了‘诵(dibuty[phthalate，DBP)攀滗簿予代大黎学习记忆缝支的影峨和海翳 spinophilin鏊闲滚这晌影响。誊萱瓣与方法；烂镞Wismr犬醚髓鄹龄成4羹l；3个幂嘲DBP剡避实验澍(25、75’ 25 mg／kg)窬1个埘熬蛆，，筹缝咎鼠詈孕第6d鬃产鼷28d龄舅《绦予DBP竣络潮辩照满搿。魏黎学鬏、仔鬣袋 辫藤的撼奉溺滗，蘩掰窕麓PCR竣测2ld龄雄愧搭阪辩舄中spinophilin黪瓣的浚怂愫隧，Morris承遴鬻测试I 翔黔雄性移鼠豹学习记忆隧内。鲶鬃：程实鲶浏辍琵溺内。孕漩光fl|i艇；p毒菠邋：黼膺鼋缎缀壤落耪藏狂髓距、缝 妖嘴照辫短《户《0．01)。藏求逑窝黛骏中。骶剡赣嬷好藏涟避滋捩潮羧对照组镌对蘧《P《0．05)，空瓣搜索试黢r静 目豁蘩限的涔餐孵翊瞧搦露黼麓泸《0。01)。鬣嗣爨缴努飘海玛spinophitin蘩阏昀淡遮鞍麓照躐。￡诩35，7％妒《 0，05)。结论：DBP蹲期瓤噙乳期鬃灞念晦慨，≯找娥靛大鼠的掌列记忆缝力，程遴spinophilin肇溯黼遴逸，磁嚣之 间葡，挠存斑⋯澎的淤累。 美懿谲：褥瑷攀；藩渗二《?l；『陵。：三。vr鹣：学习}己能；spinophilin熬嘲 Effects ofDBP on Learning and Memory and Spinophilin ExpreSSion in Hippocampus of the F 1 Generation Rats LI Yuanfeng，ZHUANG Meizhu，LI Tao，SHI Nian (Department of Health Toxicology,Tongji Medical College of Huazhong Universi绺of Science鞠d Technology，Wuhan 430030) Abstract：Background and Aim：To study the effects of dibutyl phthalate(DBP)exposure 011 leaming and memory of the F I generation rats，as well as the expression of spinophilin gene in hippocam躺． Materials and Methods：Pregnant Wistar rats were randomly divided into 3 experimental groups and control group，each treated with 25、75、225 mg／kg DBP Or vehicle only(corn oil)by gavage since gestation day 6 to I鼢stnatal day 28。The general effects of DBP on the pregnant rats and the offspring were observed。The expression level of spinophilin gene in hippocampus of the 2 1 day-old male pups WaS determined by Real Time RT-PCR，and the learning and memory ability ofthe one month-old male pups was evaluated through Morris water nI巍黛e．ResultS：No overl sign of toxicity was found in the ’鏊金璜圉t壤鬻囱然辩擘蕊淦骚渤固蓬翻(30{!；00485)。 像者简套；零元蹲，磐，攀：|_=，媾士瓣宠缓，研筑蠢国：襁妊谚疆，骖髓诱瘦绷掌惫念濑。E一㈣il：Sandre∽163．coin。 透谶于乍者#誉瀚，Tel：027E-nm：ik novembertao@163．com。 l dams，but the male pups in the high dose group showed shortened AGD and tail length(P《啦0 1)．In the water maze tests．1atency to find a hidden platform was longer in the low dose group