体外循环期间肺动脉持续氧合血灌注对肺功能及细胞间黏附分子-1表达的影响.pdfVIP

中国循环杂志 2ω8 年 6 月第 23 卷第 3 期(总第 ISI 期)Chinese Circulation 10咀mal.June.2∞8. Vol23 No. 3 (Serial No. ISI ) 221 ·基础与实验研究· 摘襄 体外循环期间肺动脉持续氧合血灌注对肺功能 及细胞间翻附分子-1 表达的影晌 袁峙，李晓峰，赵宇东，武文琦，柏松，高罔庆，李仲智 目的:探讨体外循环(CPB)期间肺动脉持续氧合血耀性对肺功能及肺组织内细胞间翻附分子-} (ICAM-l)表达的影 响。 方法:实验用健康小型猪随机分为两姐，对照组常规行 C阳，实验组行 CPB 期间肺动脉持续擅注氧合血。分别于不 同时间点留取肺组织和血搜标本，进行肺功能和肺含水率的测定以及肺组织的病理学观察，并来用逆转进聚合酶链反应 (RT-PCR) 方法检测肺组织 ICAM愉1 mRNA 的表达。 结果:与对熙、组相比，实验组肺静恋顺应性( Cstat) 、肺氧合指数(01) ，呼吸指数(阳)等肺功能指标均有显著改静 (P O. 05 - O. 01 ) ，肺食水率明显下降(P 0. 05) ，肺组织损伤程度明显减轻;实验组 ICAM-l mRNA 的表达撞去也较对照 组跟著降低(PO.OI) 。 结论 :CPB 期间组肺动脉持续握住氧合血可减轻肺损伤、保护肺功能，而 ICAM-l 可能参与了这种保护机制。 提罐词 肺:体外循环;缺血再灌注损伤;肺动脉灌注;猪 1be ElIect of ContinuOl且s Pulmooary Perfl酣ion Witb Oxyge阳能d BI制KI 08 Pulmonaη Functi佣 and IntraceUuJar Adhesíon Molecule-l Expr邸sion During CardiopulmonaηBypass YUAN Feng , U Xiao-fe吨， ZHAO Yu-dong , et al Heart Center , Beíjing Chi恤回， s Hospital AffiliatedωCapi时 Medical UlÚversity , Beíjing (1创阳05) ，ωm Ab脚唰 Objective: To ínvestigate the e:ffect of continuous pulmonary pe巾sion with oxygenated b切。d on p叫monary function and in- tracell叫ar adhesion mol创ule-l (ICAM-l) expres自ion during c盯diopulmonary bypωs (CPB). Methods: Healthy piglets were randomly divìded into two 伊四庐，切le control group only accepted a routine CPB , whereas 由.e experimental group receíved contínuous pulmonary perfusìon with oxygenated blood simultaneously with CPB. Lung tissue and bl∞d sample we陀 obtained at varlous time points to me温.sure the pulmonary function and pulmonary water content , and to exam峭 íne 由e histological structures 响曲 light and electron microscope. 币le expression of ICAM-l mRNA was also determined by semi- quantitative RT-PC民 R创ults: Comp创叫 with the control 伊up ， the pulmonary function w翩 sì伊ificantly ímproved in 由e experimental group as índícated from the parameters of the lung compliance , oxygenatìon index and respiratory index( P 0.05 or P 0.01).ηle water content of the lung wωmuch lower in the experimental group 出an that in the control group( P O. 05).