水稻土中硫酸盐还原微生物研究进展.pdfVIP

29 8 Vol. 29, No. 8 2009 8 ACTA ECOLOG ICA SIN ICA Aug. , 2009 1, 2 1 1, 刘新展 , 贺纪正 , 张丽梅 ( 1. , 100085; 2. , 100039) : , ( su lfate- reducing bacteria, SRB) , , SRB SRB , (T-RFLP) ( DGGE ) PCR ( real-tmi e PCR)( FISH ), SRB : ; ; ; ; ; PCR; : 1000-0933( 2009) 08-4455-09 : S154. 36 : A The sulfate-reducing bacteria and surfer cycle in paddy soil 1, 2 1 1, LIU X in-Zhan , HE J-i Zheng, ZHANG Li-M ei 1S tateK ey Laboratory of Urban and R eg ional E cology, R esearch Centerf or E co-Env ironm ental Sciences, Ch inese A cademy of Sciences, B eij ing 100085, China 2 Grad ate University of Ch ineseA cademy of Sciences, B eij ing 100039, China A cta E co log ica S in ica, 2009, 29( 8) : 4455~ 44 3. Abstract: Sulfur is one of the most necessary nutrients for rice growth. Sulfate reduction is a key process in the sulfur b iogeochem ical cycle, which is active in the surface and rhizosphere of the rice paddy soils. Sulfate-reducing bacteria ( SRB) are the m ain functional m icrobial group and can also play mi portant roles in the degradation of som e organic pollutants in the rice paddy soils. This rev iew paper summ arized recentm ajor advances in the processes of sulfate reduction and the diversity of SRB in the rice paddy soils. It also introduced som e prevalentm olecular m ethods in the stud ies ofSRB in the rice paddy soils, such as term inal restriction fragm ent length polym orphism ( T-RFLP), denature gradient gel electrophoresis ( DGGE ), rea-l tmi e PCR, and fluorescence in sit hybridization