MAPK signaling pathway mediated by rapid activation of 17-estradiol of human sperm.doc

 PAGE \* MERGEFORMAT 13 MAPK signaling pathway mediated by rapid activation of 17-estradiol of human sperm [Abstract] Objective To observe the 17-estradiol (E2) on human sperm activated rapid non-genomic effects, and explore the related mechanism. Methods estrogen receptor antagonist Tamoxifen (0.05mol / L) pretreatment, after 20min, were treated with E2 (1mol / L), estradiol - bovine serum albumin conjugate (E2-BSA, 5mol / L effect of the sperm were capable 15min, with three-color staining was detected by the genius sperm acrosome reaction rate, Western blotting, mitogen-activated protein kinase (MAPK) protein expression. Results E2, E2-BSA can improve human sperm acrosome reaction rate and the promotion of p42/p44 MAPK protein phosphorylation, and both effects were can be blocked by Tamoxifen. Conclusion membrane of human sperm membrane estrogen receptor may exist (mER), combined estrogen and mER can be rapidly activated MAPK pathway in human sperm. [Keywords:] 17-estradiol, human sperm acrosome reaction, membrane estrogen receptor, mitogen-activated protein kinase AbstractObjective To study the rapid non-genic effect and mechanism of 17-estradiol on human spermatozoon activation.Methods Human spermatozoa were cultivated with E2 (1mol / L) or E2-BSA (5mol / L) for 15 minutes after treated with tamoxifen (0.05 mol / L) for 20 minutes.Acrosome reaction rates were scored by trichrome staining and MAPK activity was determined by Western blotting.ResultsE2 or E2-BSA significantly increased the acrosome reaction rates and MAPK activity (p42/p44). The effects of E2 or E2-BSA were able to be inhibited by tamoxifen.ConclusionsThere may be some membrane estrogen receptors on the human spermatozoa, which mediate the rapid activational effects of estrogen on human spermatozoa through MAPK pathways. KEYWORDShuman spermatozoonacrosome reactionmembrane estrogen receptormitogen-activated protein kinase More and more research data shows that estrogen in sperm m