Math1 expression of hedgehog protein to promote experimental study of inner ear.doc

 PAGE \* MERGEFORMAT 18 Math1 expression of hedgehog protein to promote experimental study of inner ear Of: Hu Xiaohua, Huang Jianmin, China’s Lin [Abstract] Objective To study the hedgehog protein (Shh) on mouse cochlear pluripotent progenitor cells (CNPs) in the regulation of Math1 to explore the treatment of sensorineural hearing loss in new ways. In vitro culture methods on day 1 after birth CNPs mouse cells were cultured in DMEM/F12 + Shh by RT PCR, luciferase assay system, immune fluorescence staining and flow cytometry techniques were used to detect early cell differentiation induced by CNPs important transcription factor Math1 in the expression. Results Shh inducing the expression of Math1 mRNA group, Math1 promoter transcription and protein expression of Math1-positive rate higher than that induced (P lt;0.05); inhibitor LY294002 significantly reduced Shh transcription of Math1 promoter and The role of Math1 expression (P lt;0.05). Conclusions day 1 after birth in mice CNPs cells the induction of Shh can promote the transcription and expression of Math1 to induce early differentiation of cochlear hair cells. [Keywords:] gene expression in cochlear hair cells trans-activating factor type sensorineural hearing loss of reverse transcription polymerase chain reaction Most sensorineural hearing loss of hair cells from the spiral and its associated nerve damage and degradation. Cochlear hair cell progenitors present in the mammalian cochlea, the hearing loss and have to replace the potential of hair cells, However, these cells in a quiescent state, and not spontaneous proliferation or differentiation of hair cells [15]. has not yet found the inner ear hair cells in mammalian phenomenon of spontaneous regeneration. Although so far many studies have been carried out at home and abroad, but not found to promote inner ear hair cell regeneration and effective method. In this study, in vitro mouse cochlear pluripotent progenitor cells (cochlear neural