Matrine on BALB - c immune protective effect of liver injury in mice.docVIP

 PAGE \* MERGEFORMAT 10 Matrine on BALB / c immune protective effect of liver injury in mice [Abstract] Objective matrine on BCG (BCG) and lipopolysaccharide (LPS) induced liver injury animal model in rats. Methods immune BCG + LPS induced liver injury model, modeling 2 days gavage to matrine (Mat) and DDB (Bif), a total of 10d, calculated liver index, spleen index, the liver tissue spectrophotometry catalase glutathione (GSH- Px), total antioxidant capacity (T-AOC) and malondialdehyde (MDA). the results of mice by oral administration of matrine 20,40,80 mg / kg, can significantly reduce the liver, spleen index (P lt;0.05, P lt;0.01), decreased MDA content in liver homogenate, the decrease of liver homogenate GSH-Px, T-AOC activity increased (P lt;0.05). Conclusion of matrine on immune liver injury in mice showed a protective effect The mechanism may be related to enhanced antioxidant activity. [Keywords:] matrine immunological liver injury in BCG LPS Matrine (matrine, Mat) is a species from the legume Cassia Sophora (Sophora alopecuroides L) in the alkaloid extract, have anti-inflammatory, anti-virus, enhance immunity, protect liver function. According to the literature, Mat on CCl4 induced liver injury in animal models of liver cells in rats [1], but has yet to see matrine on BCG (BCG) + lipopolysaccharide (LPS) induced BALB / c mice with reports of liver injury . In this experiment, BALB / c mice as experimental subjects, of matrine on immune liver injury in BCG + LPS in rats. 1 Materials and methods 1.1 Animals BALB / c mice, male, 6 to 8 weeks old, clean, by the Experimental Animal Center of Ningxia Medical University, Certificate of Conformity: SCXK (Ning) 2005-0001, at room temperature (21 ~ 23 ), normal light environment after 1 week feeding experiment, the standard diet, free access to water. 1.2 drugs were purchased from Ningxia Bauhinia Mat Pharmaceutical Co., Ltd., batch number: white crystal, DDB IMC purchased from Zheji