Mesangial proliferative glomerulonephritis in children with Bax protein expression and apoptosis in peripheral blood lymphocytes.doc

 PAGE \* MERGEFORMAT 15 Mesangial proliferative glomerulonephritis in children with Bax protein expression and apoptosis in peripheral blood lymphocytes [Abstract] Objective clearly pathological mesangial proliferative glomerulonephritis (mesangial proliferative glomerulonephritis, MsPGN) The primary nephrotic syndrome (nephrotic syndrome, NS) in children with peripheral blood lymphocytes (peripheral blood lymphocytes, PBLs) Bax protein expression and apoptosis in PBLs. MsPGN-NS children of glucocorticoids (glucocorticoid, GC) resistance part of the mechanism. Methods All patients and normal children were taken in the morning under fasting venous blood by density gradient centrifugation, PBLs, derived cells with phytohemagglutinin (phytohemagglutinin, PHA) or PHA +1 10-6mol / L dexamethasone (dexamethasone, Dex) treatment, 24 h, and 48 h after the application of flow cytometry Bax protein expression and apoptosis rate. Results MsPGN-NS children and normal children Dex group PBLs Bax protein expression were significantly higher than the control group (P lt;0.01), and time-dependent manner (48 hgt; 24 h ), but each time MsPGN-NS patients the expression of Bax Dex group were lower than the corresponding group of normal children (P lt;0.01); MsPGN-NS Dex group of children and normal children were higher than PBLs apoptosis in the control group ( P lt;0.01), and time-dependent manner (48 hgt; 24 h), but each time MsPGN-NS cells in children with Dex group were lower than normal children group (P lt;0.01). Conclusion MsPGN-NS patients children PBLs Bax protein levels and PBLs was significantly correlated apoptosis, but its expression was significantly lower than the control group, suggesting that low expression of Bax protein leading to apoptosis in PBLs of children MsPGN-NS reduced the reason and cause GC resistance one. [Keywords:] mesangial proliferative glomerulonephritis; nephrotic syndrome; peripheral blood lymphocytes; Bax; apoptosis ) Abstract: Oje