Mesoporous SBA 15 fixed amount of fat determination of enzyme.doc

 PAGE \* MERGEFORMAT 14 Mesoporous SBA 15 fixed amount of fat determination of enzyme Of: Shang Yan Li Qin Xiaohuan Lubao Wang Qiang Fu [Abstract] absorption method using Candida lipase (candida rugosa lipase, CRL) fixed on mesoporous SBA 15, compared to a single wavelength by spectrophotometry, dual wavelength ultraviolet spectrophotometry and two cinchonine acid ( bicinchoninic acid method, BCA) method for the determination of the enzyme protein concentration and a fixed amount of enzyme protein. The results show that: SBA 15 on the UV absorption of significant interference, the results of a single wavelength much larger than the determination in dual-wavelength ultraviolet method and the BCA method, dual-wavelength ultraviolet method and the results closer to the BCA method. BCA method using different concentrations of CRL mesoporous molecular sieve in a fixed amount of immobilized enzyme investigated the leakage. in the number and LLSD1 were Lu001 the mesoporous molecular sieve SBA 15 on the set enzyme were 16.6 and 114.12 mg / g. In the buffer solution SBA 15 of the immobilized enzyme leakage rate is only 0.5%, as well as enzyme immobilization carriers. [Keywords:] mesoporous SBA 15, lipase, a fixed amount of enzyme protein, ultraviolet spectrophotometry, the second acid cinchonine 1 Introduction Mesoporous materials with large pore diameter ranging between microporous, with a large surface area and the multi-dimensional pore structure of mesoporous silicon-based molecular sieve SBA 15 straight with a highly ordered hexagonal pore structure, pore size in the range of 5 ~ 50 nm, the enzyme molecule has a strong adsorption capacity, the new material is immobilized [1]. the enzyme fixed on mesoporous molecular sieve activity conducive to maintaining stability, ease of reuse of the enzyme. high and low fixed amount of enzyme leakage of enzymes volume is an important indicator immobilized. mesoporous zeolite fixed amount of enzyme determination w