Mesenchymal stem cells in vivo experimental study of osteogenesis.doc

 PAGE \* MERGEFORMAT 25 Mesenchymal stem cells in vivo experimental study of osteogenesis Author: Li Zhang Hua, Hao Peng, Liao, Yu-Fu Zhang, Qiang Zhao, Chang-Yong Keywords: Tissue engineering of bone Abstract: [Objective] To investigate the mesenchymal stem cells in vivo osteogenic potential. [Methods] taken from the sheep iliac crest at 10 ~ 15 ml bone marrow using Percoll gradient separation medium by centrifugation to isolate mesenchymal stem cells, cultured, proliferation after planting in a porous β tricalcium phosphate on bone tissue engineering , implanted in eight sheep, the left hind metatarsal defect area (length 21 mm) as experimental group; pure β tricalcium phosphate implanted porous ceramic material 8 sheep as a control group; 6,12,24 weeks after surgery, respectively animals were killed OK radiology, histology and biomechanical testing. [Results] radiology and histology testing, the experimental group after 6 weeks, you can see there are new bone formation in the control group had no obvious new bone formation; bone defect site new bone-like tissue, woven bone and bone plate appearance time of experiment Groups are also earlier than the control group, and not mediated by the cartilage that can directly into the bone, while the control group from both ends of “creeping substitution” approach into the bone. After 24 weeks, radiological and biomechanical testing of bone defects in the experimental group showed almost complete restoration, the control group only part of the healing. [Conclusion] The mesenchymal stem cells not only have good in vitro proliferative capacity, return to the body after implantation into the bone still has a good ability to eliminate the need for the course will be able to direct the formation of new cartilage bone, showing the mesenchymal stem cells as bone tissue engineering seed cells a good prospect. Keywords: Tissue engineering of bone; bone defect; new bone; mesenchymal stem cells, Abst