Mesenchymal Stem Cells.docVIP

 PAGE \* MERGEFORMAT 14 Mesenchymal Stem Cells [Abstract] Mesenchymal stem cells Stem cell research is one of the hotspots. Although in recent years on stem cell research have made #8203;#8203;great progress, but there are still many issues to be further addressed. This paper mainly mesenchymal biological characteristics of stem cells and immune tolerance, differentiation and promote the repair, mesenchymal stem cell markers and other issues were reviewed. [Keywords:] bone marrow mesenchymal stem cell differentiation marker Mesenchymal stem cells (bone marrow derived Mesenchymalstem cells, BMSCs) in addition to bone marrow hematopoietic stem cells (hematopoieticstemcells, HSC) than in the other stem cells, bone marrow microenvironment is an important part of both in vivo and with the support The role of hematopoietic regulation. because of their relatively easy and the formation of adherent fibroblast-like clone, therefore, also known as fibroblast colony-forming units (Colonyforming unit fibroblast, CFU F). and because they come from bone marrow of the support structure, and as a nourishing layer supports the growth of hematopoietic stem cells, so it was also called bone marrow stromal cells (Bone marrow stromal cells, BMSCs). 1, the biological characteristics of bone marrow MSCs BMSCs cultured in different species of similar morphological features, mainly for the spindle, spindle, a small number of polygons. Currently, BMSCs separation methods include the following: (1) whole bone marrow culture, is sterile extracted bone marrow cells were added into the culture medium and cultured in primary culture to culture the majority of hematopoietic cell components, for the benefit of adherent BMSCs growth, DMEM and fetal bovine serum can be cultured. BMSCs on the high nutritional requirements, fetal bovine serum final concentration of 10% to 20%, it was considered as the replacement fluid red blood cell will gradually be naturally removed, had little e