Method of removing protein polysaccharide Chuanxiong.docVIP

 PAGE \* MERGEFORMAT 13 Method of removing protein polysaccharide Chuanxiong Of: Fang Sheng-ping, Wang Wei Hong, Luo dragons [Abstract] Objective Chuanxiong polysaccharides in addition to the protein treatment, the best screening method for removing protein. Methods Sevag method, trichloroacetic acid, TCA - 3 methods butanol method of removing protein processing Chuanxiong polysaccharides, by comparing the different methods treatment, the protein and polysaccharide content to determine the best method of removing protein. Results Sevag method of protein removal efficiency (90%), but the recovery rate of only 40% of the polysaccharide; trichloroacetic acid method of protein removal was 37.28%, polysaccharide recovery was 73.19%; trichloroacetic acid - butanol method of protein removal rate was 83.60%, polysaccharide recovery was 85.16%, and the operation is quick and convenient. Conclusion trichloroacetic acid - butanol method of eliminating protein effect was good. [Keywords:] Chuanxiong; polysaccharide; addition to protein Chuanxiong as lt;”China Pharmacopoeiagt;” -2005 edition (I the Ministry) gathers varieties, Chuanxiong Umbelliferae Ligusticum chuanxiong Hort.’s Dried rhizome, with blood circulation of qi, chills and pain in effect, often used to treat irregular menstruation, amenorrhea dysmenorrhea, abdominal pain, Zheng Jia, Xiong Xie tingling, or flutter swelling, pain, headache, Fengshibitong [1]. Chuanxiong containing lactones, alkaloids, phenols, and volatile oils and other compounds, their Polysaccharides also gradually attention [2,3]. In view of polysaccharides constitute one of the basic material of life, with a variety of physiological activity [4], on Chuanxiong polysaccharide is particularly necessary. System Chuanxiong sugars, to clarify its biological activity, for the development of high-tech health food and drug Chuanxiong polysaccharides provide a theoretical basis. Which is a purified polysaccharide Chuanxiong first and cr