Methylmercury chloride anti-U251 glioma cells in nude mice in vivo study.doc

 PAGE \* MERGEFORMAT 11 Methylmercury chloride anti-U251 glioma cells in nude mice in vivo study Of: Liu Ying Chen Xuan Bi Xiaoying Li Chao Yuan Changji [Abstract] aims at developing a subcutaneous U251 glioma model in nude mice, based on morphology by in vivo experiments and related research, analysis of methylmercury chloride (MMC) of the anti-glioma effect. U251 nude mice were prepared by plastic stromal tumor were randomly divided into control and experimental groups, the experimental group of nude mice injected with U251 glioma cells after 1 w, daily intragastric administration of 10 mg / kg body weight MMC, consecutive 5 d, control group received the same volume saline. All mice in the tumor were killed 14 d after inoculation. pathologic results of the experimental group was significantly lower than the control group tumor size, histological observation, they showed that the growth of U251 glioma cells in lower density than the control group, and visible cells smaller size, nuclear condensation and other morphological changes of apoptosis. Transmission electron microscopy showed that the experimental tumor cell nuclear condensation, chromatin surrounding technology, nuclear fragmentation, cytoplasmic vacuolation and other changes in the experimental group were significantly tumor Mercury the control group. Conclusion MMC U251 glioma cells in nude mice significantly inhibited, which may be realized by inducing apoptosis of glioma cells, the associated molecular mechanism needs further study. [Keywords:] methylmercury chloride; glioma; cell proliferation; cell apoptosis [Abstract] Objective To study the morphology change in apoptosis effect of methyl mercuric chloride (MMC) on brain neuroglioma. Methods Experimental group were lavaged with MMC for 5 d and 10 mg / kg BW per day after the conduction of the athymic rat model of neuroglioma, control group was given with sodium chloride at the same dose. Results Oncopathology study showed that the av