Microwave Method for Rapid Extraction of Genomic DNA of filamentous fungi.doc

 PAGE \* MERGEFORMAT 6 Microwave Method for Rapid Extraction of Genomic DNA of filamentous fungi Authors: Yong-Qiang Tian Su Min Zhao Honglin Chen Xiaolin Yang Shao Yang Zuo Zhao Lixin Feng Zhao Zaihan Ho Chu-Wen [Abstract] filamentous fungal genomic DNA extraction is a time-consuming, laborious and expensive work, and less efficient. This paper reports the microwave thermal vibration of a broken deal with fungal hyphae, fast, simple and efficient extraction method of fungal genomic DNA. To extract the DNA as a template can be amplified 18S rRNA genes. [Keywords] microwave method; filamentous fungi; genomic DNA extraction; Filamentous fungus widespread in nature, in the industrial and agricultural production accounts for a very important position [1]. The development of filamentous fungi requires a large number of bacteria for rapid screening, identification and improvement. The development of molecular techniques for rapid and efficient identification of fungi and improvement become a reality, so quick, convenient and affordable fungal DNA extraction becomes important. Because there is different from the fungal cell wall of bacteria and fungi polysaccharides have more impact on the quality and follow-up made by DNA analysis. Extraction of the usual methods, such as actinomycetes, bacteria, DNA method is not suitable for extraction of fungal genomic DNA, while the commonly used method of enzymatic or liquid nitrogen broken in practice because of cost and storage conditions of the problem has been limited to applications. Many scholars of the filamentous fungi genomic DNA extraction methods were explored and improved [2,3]. Microwave oven has become a laboratory, one of the conventional devices, Xu equality [4] using microwave extraction of DNA actinomycetes get better results. This has inspired us to microwave method applied to the extraction of fungal genomic DNA, to find a new filamentous fungal genomic DNA extraction method. 1 Materials and methods 1.1