Minus non-frozen preservation L02 cells and apoptosis in the effect of the relationship between.doc

 PAGE \* MERGEFORMAT 18 Minus non-frozen preservation L02 cells and apoptosis in the effect of the relationship between Study: New Li Qing-Yong Li Qing-Hua Zhang Jiang Zhi Li Li Chen Ling Gao Yi Security Xia Aixiang [Abstract] Objective UW solution minus non-freezing (-0.8 preservation of biological artificial liver cells with L 02, and conventional low temperature (4 and 0 compared with non-frozen preservation of minus explore the effect of liver cells and with cells relationship. UW solution prepared well preserved L 02 cell suspension divided into 3 groups: -0.8 group (minus non-freezing group, 0 group (0 non-freezing group, 4 group (the control group. cryopreserved 24 h, 48 h and 72 h, cell viability and apoptosis were determined rate (flow cytometry, LDH release, urea synthesis, albumin secretion. minus the result of non-freezing (-0.8 higher than normal low temperature (0 and 4 cryopreservation significantly increased the survival rate of cells in L 02 (72 h: -0.8 (70.17 + -2.82% vs 4 (60.05 + -3.17%), reduced the apoptosis rate (72 h: -0.8 (5.73 + -1.68% vs 4 (9.20 + -2.35%). subzero non-freezing significantly inhibited the LDH release (72 h: -0.8 (113.88 +-5.64U / L vs 4 (170.47 +-11.80U / L), L 02 to better maintain the cell’s synthesis of urea (72 h: -0.8 (1.01 +-0.14mmol / L vs 4 (0.66 +-0.09mmol / L) and albumin secretion (72 h: -0.8 (9.04 + -0.53g/ml vs 4 (7.70 + -0.52g/ml). Conclusions and compared to 0 and 4 , minus non-freezing (-0.8 L 02 significantly increased cell survival and reduce apoptosis induced by low temperature injury, the effective protection of liver cells secreted urea and albumin synthesis function. [Keywords:] bioartificial liver, minus non-icing [Abstract] Objective To investigate the effect and the relationship with cell apoptosis of subzero nonfreezing storage (-0.8 ) compared with conventional hypothermic storage (4 and 0 using L 02 hepatocytes which were stored in UW solution and used by