Model of hibernating myocardium in rabbit bone marrow stem cell mobilization and myocardial vascular endothelial growth factor gene expression.doc

 PAGE \* MERGEFORMAT 8 Model of hibernating myocardium in rabbit bone marrow stem cell mobilization and myocardial vascular endothelial growth factor gene expression [Abstract] Objective: To evaluate the rabbit model of hibernating myocardium changes in peripheral blood and bone marrow stem cells in ischemic myocardial tissue of vascular endothelial growth factor gene expression changes. Methods: Male Japanese white rabbits as the research object, through the open-chest ligation of coronary insufficiency left anterior descending artery method to establish animal model of hibernating myocardium. will be successfully prepared in 24 rabbits were randomly divided into four groups, namely, ischemia 3 d group, ischemia-7 d group, ischemic 28 d group and sham-operated control group. Application model of each group detected by flow cytometry in peripheral blood mononuclear cells in animal cells, the percentage of CD34, using real-time fluorescence quantitative PCR in each group were determined animal models of ischemic myocardial tissue of vascular endothelial growth factor mRNA. Results: The rabbit model of hibernating myocardium in the surgery after bone marrow stem cell mobilization, 1 wk and reached the peak within, gradually weakened with time; a corresponding appeared within 1 wk after surgery in ischemic myocardium of vascular endothelial growth factor mRNA expression was significantly increased expression gradually decreased with time. Conclusion : hibernating myocardium can be through the organization of local vascular endothelial growth factor expression increased the mobilization of bone marrow stem cells into the peripheral blood. [Keywords:] myocardial stunning bone marrow stem cell mobilization of vascular endothelial growth factor 0 Introduction Stem cell therapy of ischemic heart disease in animal experiments and small-scale clinical trials have made great progress [1-2]. The current research in this area focused on two aspects, namely, acute myocar