Monocyte-derived foam cells in the expression of TREM1.doc

 PAGE \* MERGEFORMAT 10 Monocyte-derived foam cells in the expression of TREM1 [Abstract] Objective To investigate myeloid cell triggering receptor 1 (TREM 1) in oxidized low-density lipoprotein cholesterol (ox LDL) induced macrophage-derived foam cells. Methods successively with PMA, ox LDL induced human U937 cells, it transformed into foam cells. by RT PCR method and Western blot PMA induced group, low-density lipoprotein (LDL and ox LDL group of cells in the TREM 1 mRNA and protein expression. Results and PMA induced group , PMA + LDL group were compared, PMA + ox LDL cells TREM 1 mRNA and protein were significantly increased (P lt;0.05. Conclusion TREM 1 may be involved in foam cell formation, and atherosclerosis (AS plaque development is closely related to the treatment of AS may become a new target or monitoring the progress of a new index AS. [Keywords:] foam cells, myeloid cells triggering receptor 1, atherosclerosis Foam cells of atherosclerosis (AS plaques characteristic pathological cells appear, mainly from blood monocytes in the membrane of vascular smooth muscle cells. More and more together to form the lipid accumulation of foam cells and even the fat stripe quality patches. U937 is a human lymphoma cells, in essence, monocytes, in vitro cell cultures were suspended growth, stimulated by phorbol ester can differentiate into macrophage-like cells, which oxidized LDL containing lipoprotein cholesterol (ox LDL in the culture medium can be transformed into foam cells (1). myeloid cell triggering receptor 1 (triggering receptor expressed on myeloid cells 1, TREM 1 is a 30 ku glycoprotein, can induce a variety of inflammatory factors and chemical factors such as tumor necrosis factor-@ (TNF @, interleukin 8 (IL 8 and monocyte chemoattractant protein 1 (MCP 1 generation. Thus, TREM 1 in the regulation of neutrophil and monocyte activation and inflammatory response in a more important position. but the TREM 1 in monocyte-derived foam cells, the