Morchella Study of extraction conditions.doc

 PAGE \* MERGEFORMAT 12 Morchella Study of extraction conditions [Abstract] Objective To study the best way Morchella polysaccharide, and to compare different kinds of morels in the difference between the yield of polysaccharides. Methods Morchella GIM5.69 [(Morchella esculenta (L.) Pers.)] and black veins Morchella GIM5.29 (Morchella elata) mycelia as subjects to study, compared with conventional hot water extraction methods, ultrasonic and the yield of enzyme treatment on the different effects of polysaccharide, and compare the two types of Yang Du bacteria on the difference between the yield of polysaccharides. Results Ultrasound and enzyme could significantly increase the yield of polysaccharides, which enhance the effect of ultrasound The most obvious 10 min. different types of bacteria exist in the polysaccharide yield significant differences, GIM5.69 type strain polysaccharides was significantly higher than GIM5.29 type. Conclusion esculenta polysaccharide extraction and isolation conditions were: ultrasound 10 min, ultrasound frequency 40 kHz, at 90 ℃ constant temperature heating 150 min, extracted 2 times. [Keywords:] morel, black morel pulse, polysaccharides, extraction Abstract: Objective To optimize the extraction technology of Morchella polysaccharide. Methods With GIM5.69 (Morchella esculenta (L.) Pers.) And GIM5.29 (Morchella elata) as the research object, the difference of polysaccharide yield with lixiviation in polysaccharide extraction between ultrasound and enzyme was observed. At the same time, the difference of polysaccharides yield of these two Morchella was compared. Result Ultrasound and enzyme both can improve the polysaccharides yield, and the effect of 10 min of ultrasound was the best. The polysaccharides yield of GIM5.69 was higher than that of GIM5.29, which means different strain has different polysaccharides yield. Conclusion The best extraction condition is 10 min of ultrasound at the frequency of 40 kHz, heating 150 m