Moutan Ethanol Extraction Process.doc

 PAGE \* MERGEFORMAT 10 Moutan Ethanol Extraction Process 【Abstract】 Objective: To explore the optimum conditions of extraction moutan. Methods: The orthogonal experimental method in order to paeoniflorin content indicators, high-performance liquid method. Results: The extraction times and ethanol concentration on the extraction has a very significant and significant impacts. Conclusion: The optimum conditions were 5 times the amount of 75% ethanol extract three times, each time 1h. Keywords: moutan; orthogonal design; extraction process; paeoniflorin; high-performance liquid chromatography Moutan for the Ranunculaceae plant peony (Paeonia suffruticosa Andr.) Dry root bark, with heat cooling blood, promoting blood circulation effect. Peony root bark contains paeoniflorin, oxidation paeoniflorin, benzoyl paeoniflorin, paeonol, paeonol glycosides, paeonol original paeonol new glucosides glycosides and other ingredients. Pharmacological experiments show that paeoniflorin vitro and in vivo can inhibit ADP or collagen-induced platelet aggregation [1], should be moutan anti-ischemic stroke, one of the main active ingredient. In this paper, orthogonal experimental method to HPLC Method for the Determination of paeoniflorin extraction as an index of the optimal extraction process of ethanol moutan for the development and application of moutan provide an experimental basis. An instrument and reagent High-performance liquid chromatography: Shimadzu 10Avp high-performance liquid chromatography (LC  10Atvp pairs of pumps, SPD  M10Avp diode array detector, SIL  10Advp autosampler, CTO  10Avp oven); Hypersil ODS column packing (ID4.6 × 250mm) (Dalian Elite Chemical Instruments Co., Ltd.); analytical balance: LIBROR L  16ODTP analytical balance (Shimadzu), AE240 analytical balance (Switzerland METTLER); paeoniflorin reference substance (0736  9710): Chinese pharmaceutical and biological products (for the determination of use). Mouta