MSP on schistosomiasis liver fibrosis factor expression.doc

 PAGE \* MERGEFORMAT 11 MSP on schistosomiasis liver fibrosis factor expression [Abstract] Objective To observe the MSP mice liver fibrosis in schistosomiasis hyaluronic acid (HA), serum procollagen type Ⅲ (PC Ⅲ), hydroxyproline (Hyp) and transforming growth factor-β1 (TGF-β1 ) mRNA expression. Methods NIH mice infected schistosome cercariae through the skin were randomly divided into 4 groups: model group (B group), praziquantel group (C group), praziquantel + MSP group (D group), praziquantel + colchicine group (E group), while the normal control group (A group). pathologic liver tissue in mice, in liver fibrosis after 21 day gavage of the appropriate drugs and saline , C, D, E group at 45 days when the insecticide to be fed once with praziquantel, the first 78 days of mouse heart blood serum by radioimmunoassay PC Ⅲ and HA content of the liver tissue Hyp content measured; RT -PCR, the expression of TGF-β1 mRNA. Results HA levels of group D was higher than A, than B, C, E group (P lt;0.01, P lt;0.05); PC Ⅲ level of D group was lower than B, C, E group (P lt;0.05); Hyp levels of D group than in A group and less than B, C, E group (P lt;0.05); TGF-β1 mRNA expression in D group than in B, C, E group (P lt;0.05). Conclusion MSP can improve the United praziquantel schistosomiasis liver fibrosis, reducing the expression of TGF-β1 mRNA. [Keywords:] schistosomiasis; liver fibrosis; MSP; hyaluronic acid; serum procollagen type Ⅲ; hydroxyproline; transforming growth factor-β1; mice Abstract: Objectives To evaluate the effect of Modified Sini Powder on the hepatic expression of hyaluronic acid (HA), procollagen peptide (PC Ⅲ), hydroxyproline (Hyp) and transforming growth factor-β (TGF-β1) in a murine model of liver fibrosis induced by Schistosoma japonicum infection. Methods The NIH mice were successfully induced to liver fibrosis by infection with Schistosoma japonicum and then randomly divided into model group (group B), praziquantel group (group C), prazigua