Multidrug-resistant Mycobacterium tuberculosis gene mutations in resistance detection.doc

 PAGE \* MERGEFORMAT 11 Multidrug-resistant Mycobacterium tuberculosis gene mutations in resistance detection [Abstract] Objective To investigate the multi-drug resistant tuberculosis (MDR-TB) clinical isolates rPOB, KatG, and rpsL gene mutations in drug resistance testing in the application. Methods Polymerase chain reaction - single strand conformation polymorphism (PCR-SSCP) were analyzed by 54 at the same time resistant to isoniazid (INH), rifampicin (RFP), streptomycin (SM) of multi-drug-resistant clinical isolates and 45 drug-susceptible strain rPOB, KatG, rpsL mutation. Results The Mycobacterium tuberculosis H37RV as control, 45 drug-sensitive strains of rPOB, rpsL gene map of a normal SSCP and its specificity was 100%; KatG gene mapping two exceptions, the specificity was 95.6%. 89 Mycobacterium tuberculosis clinical isolates were not found rPOB, KatG, rpsL gene deletion, the PCR amplification products of SSCP analysis showed that: more than 54 drug-resistant clinical isolates, 49 rPOB genome anomaly; 32 KatG gene pattern anomaly; 40 rpsL gene mapping exception. The sensitivity of rPOB (90.7%), KatG (59.3%), rpsL (74.1%). Conclusion Mycobacterium tuberculosis resistant to RFP, INH, SM development of drug resistance was mainly due to rPOB, KatG, and rpsL gene mutation. PCR-SSCP technique is expected to become drug-resistant Mycobacterium tuberculosis detection methods. [Keywords:] Mycobacterium tuberculosis; rPOB gene; KatG gene; rpsL gene; SSCP; drug resistance Studies of Mycobacterium tuberculosis multi-drug resistant gene [Abstract] Objective To evaluat the importance of rPOB, KatG and rpsL gene mutation detection in multi-drug resistant clinical isolates of Mycobacterium tuberculosis. Methods rPOB, KatG and rpsL gene mutation of 54multi-drug resistant clinical isolates, which is INH RFP and Sm resistance and 54 drug susceptible strains were analyzed using polymerase chain reaction-single strand conformation (PCR-SSCP). Results SSCP pattern