MRC-5 cells formaldehyde-induced adaptive response of protein research.doc

 PAGE \* MERGEFORMAT 15 MRC-5 cells formaldehyde-induced adaptive response of protein research Authors: Li workhouse, ZHUANG, Liu Jianjun, Xiao-Hua Yang, Wei QIN Zhi, Huang Haiyan Keywords: formaldehyde; adaptive response; proteome Abstract: Objective To study the formaldehyde-induced adaptive response of eukaryotic cells and its possible mechanism. Methods of formaldehyde to stimulate the human embryonic lung fibroblast (MRC-5) adaptive response model; AlamarBlue reduction rate detected using cell proliferation. Two-dimensional gel electrophoresis separation of total cellular protein, image analysis, differences between selected protein spots identified by mass spectrometry. Results AlamarBlue test results showed that low doses of formaldehyde pre-stimulation can induce cell adaptive response. Two-dimensional gel electrophoresis results showed that formaldehyde-stimulated changes in 19 protein spots, peptide fingerprinting and peptide sequence tags were identified of which 13 protein spots have been identified proteins, including nucleotide diphosphate kinase, elongation factor 1 -- γ, triosephosphate isomerase, 60S acidic ribosomal protein P0, 75kDa heat shock protein, 70kD heat shock protein-like binding protein, calcium-dependent phospholipid-binding protein I, hypothetical protein FLJ34423, microtubules - actin-cross-linked factor 1, lamin B2, ATP synthase α chain, proteasome subunit α 6, these proteins function related to transcription regulation, protein folding, signal transduction, energy metabolism, cytoskeleton and other aspects. Conclusion Low doses of formaldehyde can induce the adaptive response of MRC-5 cells, a variety of proteins involved in cell regulation and control of the adaptive response. Keywords:: formaldehyde; adaptive response; proteome Proteomie analysis of adaptive response induced by low concentration of formaldehyde in human embryonic fibroblasts cells Abstract: Objective To study the adaptive re